Parentage verification is based on the comparison of genetic information from Offspring, Dam and Sire. The basis of parentage verification can be used in many species, including animals, birds, fish and humans. Sample material such as hair roots, swabs, semen, and several other materials can be used for parentage verification. Of each sample, a DNA-profile is established using DNA-markers. Two types of markers are available to perform parentage verification: STR (microsatellite) markers and SNP markers. Both are based on different technologies and the results are not comparable to each other. In case you are not familiar with the difference between these two types of marker sets, please contact us before ordering a test to determine which marker type is needed to perform the parentage verification.
The DNA-profile from each sample is stored in a database and can be presented as barcode. A barcode is unique for an individual. The process of parentage verification involves a comparison of the genetic information present in an offspring with the DNA present in the possible parents. For the confirmation of a parentage, all genetic information of an Offspring must be present in the combination of Dam and Sire. In most parentage verifications, the probability of parentage is given with at least 99,5%. In addition to parentage verification, a DNA-profile can be used to verify the identity of an individual. In this case, the reliability of the identity analysis is extremely high, because all genetic information in two samples must be identical.
Test specific information
Parentage analysis based on DNA can be done at any moment in life. A DNA-profile does not change during life. The assessment of a DNA-profile can be done using virtually every material that includes cellular and nuclear material.
The Turnaround Time (TAT) depends on various factors, such as the shipment time of your sample to the test location, the test method(s) and whether the tests are performed completely or partially by a Partner Lab or Patent owner.
The TAT of tests performed at our facilities is normally 10 working days after receipt of the sample at the testing laboratory (VHL, VHP or Certagen). For tests performed by a Partner Laboratory (so-called "partner lab test") or patent owner, the TAT is at least 20 working days after receipt of your sample. Because the shipment time to our Partner Labs or patent owner may vary due to factors we cannot influence, the mentioned 20 working days are therefore an estimate.
Sometimes it is necessary to re-run your sample. We call this a retest. In that case, the TAT will of course be extended.
Location of disease or trait
Parentage verification based on DNA can in principle be performed using any material that contains cells with a nucleus.
For this test samples from all breeds are accepted.
For this DNA test we accept the following materials: Blood EDTA, Blood Heparin, Swab, Semen, Tissue. Please contact Dr. Van Haeringen Laboratorium if you wish to submit other material as listed.
The result of a parentage verification or control of identity is restricted to the questions asked. In routine cases, the conclusion is based on a large number of DNA-markers. This results in a quality of more than 99 percent if both parents are present. The reliability of a parentage verification is reduced if parents are missing or need to be reconstructed. Also, the quality of the test is influenced by the genetic variation within a population.
In parentage verification DNA-markers are visualized, in which a DNA-marker has two genetic variants. One genetic variant is paternal in origin, whereas the other genetic variant is maternal. It is possible to assess if any genetic variant is present in an offspring that is not detected in a parent. Visualizing sufficient DNA-markers will provide a good reliability to parentage verification.
Severity of Disease
Parentage verification, kinship analysis and/or control of identity are no disease.